A) the use of expression vectors to modulate the expression of a gene in a specific tissue.
B) the use of reporter genes.
C) a critical step in the generation of cDNA libraries.
D) a type of mutagenesis.
E) the production of pharmaceuticals in farm animals or plants.
G) C) and D)
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A) DNA polymerase and DNA ligase
B) RNA polymerase and DNA ligase
C) Restriction endonuclease and DNA polymerase
D) Restriction endonuclease and DNA ligase
E) Reverse transcriptase and restriction endonuclease
G) B) and D)
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A) All restriction endonucleases generate fragments with blunt ends.
B) Hydrogen bonds between base pairs are very strong.
C) Blunt ends are exposed bases that can hybridize with complementary sequence fragments.
D) Sticky ends prevent DNA ligase activity.
E) Restriction endonucleases cut both strands of DNA simultaneously.
G) A) and E)
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Correct Answer
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